Казанский институт биохимии и биофизики КазНЦ РАН

  

The Laboratory of Molecular Bases of Pathogenesis

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Head of the Laboratory

Chernov Vladislav M.
D.Sc. (Protection of Plants), professor.
Tel.: (843)2927347, (843)2319026
e-mail:  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.









Lab members:

  • Leading researcher:

1. Chernova Olga A., D.Sc. (Biochemistry), professor,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.

  • Senior researchers:
2. Davydova Marina N., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
3. Ryazantseva Irina N., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
  • Researchers:
4. Shaymardanova Gulnara F., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
5. Trushin Maxim V., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
6. Nesterova Tatyana N., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
  • Junior researchers:
7. Mouzykantov Alexey A., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
8. Baranova Natalia B., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
9. Medvedeva Elena S., Cand.Sc.,  Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.
      1. Postgraduate student:
      10. Grigoryeva Tatyana       Данный адрес e-mail защищен от спам-ботов, Вам необходимо включить Javascript для его просмотра.

        Historical Background

        In June 1987, a Group of Molecular Genetics of Plant Mycoplasmas was established at the Institute owing to the initiative of academician I.A. Tarchevsky; in June 1988, this Group was transformed into the Laboratory of Molecular Genetics of Mycoplasmas. The Declaration of the Central Committee of Communist Party of the Soviet Union and the USSR Cabinet of Ministry of August 26, 1985 (#807) – “On further development of new directions in biology and biotechnology” formed the basis for the foundation of a new Laboratory and initiation of research of the molecular-genetic fundamentals of interaction between plants and pathogenic microorganisms within the Institute.

        The choice of mycoplasmas as a model of pathogenic microorganisms was defined by the unicity of organization of the smallest prokaryotes the limited biosynthetic abilities of which did not prevent their broad distribution in the nature, overcoming the defense systems of higher eukaryotes and persistence. Much attention to mycoplasmas is given because of the practical necessity: mycoplasmas are parasites of humans, animals, plants, and main contaminants of cell cultures widely used in biotechnology. Control of mycoplasma infections and contaminations presents a serious problem the resolution of which is connected with the advantages of investigation of interaction between mycoplasmas and higher eukaryotes, and adaptation of these microorganisms to stressors.

        The first researchers of the Laboratory were V.M. Chernov and O.A. Chernova (post-graduate students of the Institute ofCytology, USSR Academy of Sciences, Leningrad), M.A. Khasanova (post-graduate student of the Institute ofBiochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Puschino-na-Oke) and an engineer Yu.V. Gogolev (from the Laboratory of Biochemistry of Cell Wall under Prof. V.V. Lozovaya).

        In 2002 the Laboratory was renamed as the Laboratory of Molecular Fundamentals of Pathogenesis. In 2003, a group of microbiologists headed by Dr. M.N. Davydova was incorporated into the Laboratory. Previously, the scientific workers of the group formed the main part of the Laboratory of Physiology and Biochemistry of Anaerobic Bacteria, founded in 1976 by an eminent professor M.I. Belyaeva. In 2006, a part of the researchers joined a Group of Molecular Biology headed by Dr. Yu.V. Gogolev.

        Much of the scientific work of the Laboratory is related to the investigation of molecular and cell biology of mycoplasmas that infect humans, animals, plants, the main contaminants of cell cultures and vaccines.

        The success of the genome projects for mycoplasmas determined the feasibility of comparative proteomic profiling of these microorganisms cultivated under stress conditions as well as of higher organisms with mycoplasmas. Studies on the genome-transcriptome-proteome analysis of mycoplasmas and eukaryotes are supplemented with the investigations of the ultrastructure of cells interacting organisms.

        The basic research topic of the Laboratory is the interaction between mycoplasmas and eukaryotes: the analysis of genomes’, proteomes’, transcriptoms’.

        Basic results for 2007-2012:

        • Members of the Laboratory for the first time performed the data of proteomic and transcriptomic analysis of mycoplasma cells cultured under different environments, as well of eukaryotes, infected with mycoplasma. As a result of these investigations, proteins and genes mediating mycoplasma response reactions to stressors, as well as those in eukaryotes after mycoplasma infection were identified.
        • It was revealed for the first time that adaptation of mycoplasmas to stress conditions was connected with intensification of the process of releasing from bacterial cells membrane vesicles - spherical membrane-bound nanostructures (diameter 20-120 nm). It was demonstrated that membrane vesicles of mycoplasmas, apart from lipids and proteins, also included genetic material and were active participants in pathogenesis. With the use of atomic force microscopy, 3D images of mycoplasma cells and secreted membrane vesicles were obtained.

        Applied research:

        The investigations on technologic application of bacterial pigments in polymer coloring in chemical industry conducted as a result, the method for utilization of bacterial pigments in technology of polyoleofine coloring has been developed and patented. Synthesis of microbial pigments was performed using intermediates of cell metabolism. It excludes expenses for multistage synthesis of pigment molecule and is recognized as the ecologically pure process.

        Work on personnel training:

        Under the guidance of the Laboratory staff for 2003-2012, the following Cand.Sc. dissertations were defended:

        • Trushin, М.V. Role of visible and infrared light in regulation of growth in Escherichia coli: abstract of a thesis: 03.00.07 / М.V. Trushin. – Kazan, 2003. – 23 p. Research manager: Prof. V.М. Chernov, Scientific advisor: Prof. О.А. Chernova.
        • Asiful, I. Ecologic characteristic of pike-perch and sander of the upper part of Kuibyshev reservoir: abstract of a thesis: 03.00.16 / I. Asiful. – Kazan, 2004. – 25 p. Research manager: Prof. V.A. Kuznetsov, Scientific advisor: Prof. V.M. Chernov.
        • Saphin, I.N. Role of mycoplasma infection in the development of acute myocardial infarction: the possible mechanisms and features of clinical manifestation: abstract of a thesis: 14.00.06 / I.N. Saphin. – Kazan, 2006. – 21 p. Research manager: Prof. I.P. Arleevskii, Scientific advisor: Prof. О.А. Chernova.
        • Mukhametshina, N.Е. Phytopathogenicity of Acholeplasma laidlawii PG8 and its adaptation to unfavorable factors:abstract of a thesis: 03.00.12; 03.00.07 / N.Е. Mukhametshina. – Kazan, 2006. – 24 p. Research manager: Prof. V.М. Chernov, Scientific advisor: Dr. Yu.V. Gogolev.
        • Muzykantov, А.А. Adaptation of mycoplasmas (Mycoplasma gallisepticum S6) to unfavorable conditions of growth: abstract of a thesis: 03.00.04; 03.00.07. / А.А. Muzykantov. – Kazan, 2008. – 20 p. Research manager: Prof. O.A. Chernova.
        • Abuzarova E.R. Features of genotypes of Helicobacter pylori and polymorphic loci of cytokine genes (IL-1 and IL-10) in patients with ulcerous disease of stomach and dodecadactylon: abstract of a thesis: 03.00.07 / Э.Р.Абузарова. – Казань, 2008. – 23 с. Research manager: Prof. O.A. Chernova. Scientific advisor: R.А. Abdulkhakov.
        • Baranova, N.B. Persistance of Mycoplasma hominis in humans: vulnerability to mycoplasma infection and mycoplasma response reactions to stressors: abstract of a thesis: 03.02.03 / N.B. Baranova. – Kazan, 2010. – 24 p. Research manager: Prof. O.A. Chernova.
        • Medvedeva, Е.S. Adaptation of Acholeplasma laidlawii PG8 to the environment: morphological, ultrastructural, pathogenic and molecular-genetic aspects: abstract of a thesis: 03.02.03; 03.01.04 / Е.S. Medvedeva. – Kazan, 2010. – 24 p. Research manager: Prof. M.N. Davydova, Scientific advisor: Prof. О.А. Chernova.

        Collaboration with:

        • FGS «SRI of Physical-Chemical Medicine» FMBA of Russia (Moscow)
        • Institute of Cytology of the Russian Academy of Sciences (St-Petersburg)
        • Kazan (Volga region) Federal University (Kazan)
        • Kazanorgsynthesis Company Limited (Kazan)
        • Kazan State Medical Academy (Kazan)
        • Engelhardt Institute of Molecular Biology, the Russian Academy of Sciences (Moscow)

        Basic publications for 2003-2012:

        1. Arleevsky I.P., Chernova O.A., Ganeeva L.A., Saphin I.N., Chernov V.M. Mycoplasma infections and myocardial infarction // Ross. Cardiol. Zhurn.-2003.-#4.-P.17-23.
        2. Chernov V.M., Gogolev Y.V., Mukhametshina N.E., Abdrakhimov F.A., Chernova O.A. Mycoplasma adaptation to biogenic and abiogenic stressful factors; Acholeplasma laidlawii nannotransformation and minibodies // Dokl. Biol. Sci. – 2004. – Vol. 396. – P. 251-254.
        3. Chernov V.M., Mukhametshina N.E., Gogolev Yu.V., Abdrakhimov F.A., Chernova OA. Adaptive reactions of mycoplasmas in vitro: “viable but unculturable forms” and nanocells of Acholeplasma laidlawii // Mikrobiologiia. – 2005. – Vol. 74, N 4. – P. 498-504.
        4. Chernov V.M., Govorun V.M., Demina I.A., Gorshkov O.V., Muzykantov A.A., Shaimardanova G.F., Chernova O.A. Adaptation of mycoplasmas to adverse growth conditions: morphology, ultrastructure, and genome expression of Mycoplasma gallisepticum S6 cells // Dokl. Biochem. Biophys. – 2008. – Vol. 421. – P. 231-234.
        5. Abuzarova E.R., Gorshkov O.V.,  Chernova O.A., Chernov V.M., Akberova N.I., Abdulkhakov R.A. Features of distribution of interleukine genotypes (IL-1 and IL-10) in patients with ulcer disease and their association with persistence of Mycoplasma hyorhinis and genotypes of Helicobacter pylori// Experimental and clinical gastroenterology.-2008.-№6.-p.27-31.
        6. Chernova O.A., Baranova N.B., Akberova N.I., Gorshkov O.V., Mouzykantov A.A., Chernov V.M. Distribution of frequencies of alleles and genotypes of polymorphic loci IL-1B, IL-1RN, and IL-10 inhumans at mycoplasma persistence (Mycoplasma hominis) // Cytokines and inflammation.-2008.-V.7.#4.- p.11-14.
        7. Chernov V.M., Chernova O.A., Gorshkov O.V., Mouzykantov A.A. Adaptation of mycoplasmas to stressful factors: nucleotide sequences nonregistered in vegetative foms M. gallisepticum S6 cells are revealed in nonculturable forms of the mycoplasma // Mol. Biol. (Mosk). – 2009. – Vol. 43, N 4. – P. 642-647.
        8. Chernov V.M., Chernova O.A., Medvedeva E.S., Sorvina A.I., Davydova M.N., Rogova M.A., Serebryakova M.V. Responses of Acholeplasma laidlawii PG8 cells to cold shock and oxidative stress:proteomic analysis and stress-reactive mycoplasma proteins // Dokl. Biochem. Biophys. – 2010. – Vol. 432, N 5. – P. 126-130.
        9. Chernov V.M., Chernova O.A., Baranova N.B., Gorshkov O.V., Medvedeva E.S., Shaymardanova G.F. Mycoplasma adaptation to stress conditions: proteome shift in Mycoplasma hominis PG37 in response to starvation and low temperatures // Mol. Biol. (Mosk). – 2011. – Vol. 45, N 5. – P. 843-851.
        10. Chernov V.M., Gorshkov O.V., Chernova O.A., Baranova N.B., Akopian T.A., Trushin M.V. Variability of the vaa cytoadhesin genes in clinical isolates of Mycoplasma hominis // NEW Microbiol. – 2005. – Vol. 28. – P. 373-376.
        11. Chernov V.M., Moukhametshina N.E., Gogolev Y.V., Nesterova T.N., Trushin M.V., Chernova O.A. Differential amplification of Acholeplasma laidlawii PG8 rrnA and rrnB nucleotide sequences during dissociation of the cell culture population// Journal of Rapid Methods and Automation in Microbiology. 2006. 14: 369-376.
        12. Chernova O.A., Trushin M.V., Mouzykantov A.A., Chernov V.M. Atomic force microscopy investigation of DNA extracted from the vegetative forms and the viable but nonculturable forms of Mycoplasma gallisepticum S6 // TheScientificWorldJOURNAL. – 2008. – Vol. 8. – P. 1104-1110.
        13. Chernov V.M., Chernova O.A., Mouzykantov A.A., Ponomareva A.A., Trushin M.V., Gorshkov O.V., Nesterova T.N. Phytopathogenicity of avian mycoplasma Mycoplasma gallisepticum S6: morphologic and ultracytostructural changes in plants infected with the vegetative forms and the viable but nonculturable forms of the bacterium // Microbiol. Res. – 2010. – Vol. 165, N 4. – P. 346-350.
        14. Trushin M.V., Chernov V.M., Gorshkov O.V., Baranova N.B., Chernova O.A. Atomic force microscopy analysis of DNA extracted from the vegetative cells and the viable, but nonculturable, cells of two mycoplasmas (Acholeplasma laidlawii PG8 and Mycoplasma hominis PG37) //TheScientificWorldJOURNAL. – 2010. – Vol. 10. – P. 894-900.
        15. Chernov V.M., Chernova O.A., Mouzykantov A.A., Efimova I.R., Shaymardanova G.F., Medvedeva E.S.,Trushin M.V. Extracellular vesicles derived from Acholeplasma laidlawii PG8 // TheScientificWorldJOURNAL. – 2011. – Vol. 11. – P. 1120-1130.
        16. Chernov V.M., Chernova O.A., Medvedeva E.S., Mouzykantov A.A., Ponomareva A.A., Shaymardanova G.F., Gorshkov O.V., Trushin M.V. Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis // J. Proteomics. – 2011. – Vol. 74, N 12. – P. 2920-2936.
        17. Chernov V.M., Chernova O.A., Mouzykantov A.A., Baranova N.B., Gorshkov O.V., Trushin M.V., Nesterova T.N., Ponomareva A.A. Extracellular membrane vesicles and phytopathogenicity ofAcholeplasma laidlawii PG8 // TheScientificWorldJOURNAL, vol. 2012, Article ID 315474, 6 pages.

        Monographs:

        • Borkhsenius S.N., Chernova .A. Mycoplasmas. Leningrad, Nauka, 1989. 156 p.
        • Borkhsenius S.N., Chernova .A., Chernov V.M., Vonskii M.S. Mikoplazmy (Mycoplasmas), St. Petersburg: Nauka, 2002.

        Patents and certificates of autorship:

        1. Borkhsenius S.N., Chernova O.A., Merkulova N.А., Smirnova Т.D. A way for detection of mycoplasma infections / certificate of autorship № 1374783. 1987.
        2. Borkhsenius S.N., Merkulova N.А., Chernova O.A. Recombinant plasmid DNA pMg16 for detection of mycoplasma infections / certificate of autorship № 1413960. 1988.
        3. Borkhsenius S.N., Chernova O.A., Merkulova N.А., Chernov V.М. A way for identification of Mycoplasma orale in cells cultured in vitro / certificate of autorship № 1559710. 1989.
        4. Chernov V.М., Khasanova М.А., Chernova О.A. Recombinant plasmid DNA рА17 used as DNA probe for detection of Acholeplasma laidlawii 118 in plant cells / certificate of autorship № 1835851. 1992.
        5. Chernova О.A., Chernov V.М., Merkulova N.А., Borkhsenius S.N. A way for identification of Mycoplasma fermentas in cells cultured in vitro / certificate of autorship № 1559709. 1989а.
        6. Chernova О.A., Chernov V.М., Merkulova N.А., Borkhsenius S.N., Shagimukhametova F.F. A way for identification of Mycoplasma salivarium in cells cultured in vitro / certificate of autorship № 1559708. 1989b.
        7. Chernova O.A., Khasanova М.А., Chernov V.М. Recombinant plasmid DNA рА1S6, used as DNA probe for detection of Acholeplasma laidlawii 185 in plant cells / certificate of autorship № 1835852. 1992.
        8. Gogolev Yu.V., Avzalov F.Z., Idrisov G.Z., Chernov V.M., Gaffarov Kh.Z. A method of detection of infected withChlamydia psittaci agricultural animals / certificate of autorship № 2135516. 1999.
        9. Kudryashov V.N., Ryazantseva I.N., Bobreshova E.E., Andreeva I.N., Valieva N.N., Ogorodnikova T.I. Polymeric coloring composition / Patent № 2420541. 2009.

        Grants:

        • Russian Fund for Basic Research 98-04-48972a. Genetic variation in mycoplasmas during their interaction with eukaryotes: the variable genome cites and ways of its re-organization.
        • Russian Fund for Basic Research 01-04-49885a. Transformation of nitric ether of cellulose by sulfate-reducing bacterium.
        • Russian Fund for Basic Research 01-04-49011a. Interaction of plants with mycoplasmas: cellular and molecular features of plant response to infection and persistence of the parasite.
        • Russian Fund for Basic Research 01-04-49008a. Polymorphism of mycoplasmas in populations of Volgaregion: the feaures of ultracytostructure and variability of genes of cytoadhesines of clinical isolates.
        • Russian Fund for Basic Research 03-04-96279 p2003 Tatarstan. Enzymes of antioxidant defence of anaerobic bacterium Desulfovibrio desulfuricans.
        • Russian Fund for Basic Research 05-04-49435a. Molecular basis of mycoplasma adaptation (A. laidlawii) to biogenic and abiogenic stressors. 2006-2008.
        • Russian Fund for Basic Research 08-04-01047a. Molecular basis of mycoplasma adaptation (M. gallisepticum) to biogenic and abiogenic stressors: proteins of nanoforms and their genes. 2008-2010.
        • Russian Fund for Basic Research 11-04-01406а. Molecular fundamentals of mycoplasma adaptation to biotic and abiotic stressors: proteins of membrane vesicles secreted by cells of Acholeplasma laidlawii PG8, and their genes. 2011-2013.
        • Russian Fund for Basic Research 12-04-31396. Formation mechanisms of resistance to antibiotics of mycoplasma: secretion of extracellular membrane vesicles and efflux of ciprofloxacin in Acholeplasma laidlawiiPG8. 2012-2013.
        • Russian Fund for Basic Research 12-04-01052a. Phytopathogenicity and secretory system of mycoplasmas: virulence factors are exported through the membrane vesicles. 2012-2014.
        • NIOKR-Tatarstan 03.3.10-163/2002-2004 (F). “Polymorphism of microorganisms associated with diseases of gastrointestinal tract in human populations of Republic of Tatarstan: features of ultracytostructure and genetic variability of Helicobacter pylori clinical isolates and mycoplasmas”.
        • NIOKR-Tatarstan 03.3.10-361/2005 (F). ‘Genetic polymorphism in human populations of Republic of Tatarstan: variability of genes of cytokines in patients with gastro-duodenal and urogenital diseases at helicobacter and mycoplasma infections”.
        • Grant by the President of Russian Federation to young scientists № МК-3372.2009.4.
        • Grant by the President of Russian Federation to young scientists № МК-4894.2010.4.
        • Grant by the President of Russian Federation to young scientists № МК-3823.2013.4.
        • Federal Purposive Program “Investigation and developments for science and technique for 2002-2006”. Government contract # 02.442.11.7283 (Principal investigator – Prof. Chernov) “Molecular and genetic mechanisms of mycoplasma adaptation to biogenic and abiogenic stressors”.
        • Federal Purposive Program ‘Living systems”. Government contract # 02.512.11.2067 (Principal investigator – Prof. Chernov) “Comparative proteome analysis of vegetative forms and nanocells of M. gallisepticum for development of technology for control of mycoplasma infections”.
        • Federal Purposive Programm ‘Living systems”. Government contract # 02.512.12.2010 (Principal investigator – Prof. Chernov): “Proteome-transcriptome analysis of mycoplasma cells and eukaryotes during their interaction for development of methods for control of the parasite-host system”. 2008-2009.
        • Program of the Presidium of RAS «Support of innovations». «Potential of secondary metabolites of microorganisms for practical application». 2008.
        • Federal Purposive Program «Scientific and scientific-educational personnel of the innovation Russia» for 2009-2013 on the lot «Performing of scientific research by collectives of scientific-educational centers in the area of technical sciences» – Governmental contract № 02.740.11.0391 «Secreted factors of toxigenicity of bacteria as promising tools for control of pathogenic processes». 2009-2011.
        • Federal Purposive Program «Scientific and scientific-educational personnel of the innovation Russia» for 2009-2013. Agreement of 20 July 2012 #8042 “Investigation of new class of secreted bacterial toxigens – extracellular membrane vesicles of mycoplasmas for development of ways for controlling mycoplasma infections, contamination of cell cultures and vaccines”. 2012-2013.