Казанский институт биохимии и биофизики КазНЦ РАН

  

The Laboratory of Biophysics of Transport Processes

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Head of the Laboratory:
Anisimov Aleksandr A., Prof.
Leading research scientist:
Velikanov Gennadii A., Prof.
Senior research scientists:
Alyabyev Alexander Ju., PhD
Belova Larisa P., PhD
Ionenko Irina F., PhD
Research scientists:
Andreyeva Irina N., PhD
Dautova Nailya R., PhD
Ogorodnikova Tatyana I.
Sibgatullin Timur A., PhD
Vorob’ev Vladimir N., PhD
Junior research scientists:
Ilyina Tatyana M., PhD
Suslov Maksim A.

Theme of the laboratory investigations:

Driving forces and regulation of water, ion, and essential element transport in plants.

Directions and tasks of investigations of the laboratory within the theme:

  • Organization of the system of water flow driving forces in plants: their mechanisms, stimuli, and coordination during climatic seasonal alterations. 
  • Study of the effect of the natural set of climatic seasonal parameters on the process of switching of the water transfer pathways and driving forces. 
  • Elucidation of the characteristic features of mechanisms of transmembrane water and ion transfer, the role of membrane contact sites in the traffic of assimilate water solutions 
  • Mechanisms of restoration of water conductivity in plant vessels after gas emboli. 

The most important achievements of the laboratory during the period 2008-2013.

The sensitivity of the apparent coefficient of water diffusion via membranes dependence of aquaporin functioning on the influence of a number of abiotic factors and on the presence of Ca2+ ions in the medium is shown, and the temperature optimum for aquaporin functioning is determined.

(Ionenko et al., Environ. Exp. Bot. 2012; Ionenko et al., Biologia Plantarum 2006, 2010; Ionenko and Anisimov, Rus. J. Plant Physiol. 2007, 2008)

Within the study of regulation of intercellular water transfer by means of hydrodynamic stimuli, alterations in the endomembrane system are shown to occur under pressure values close to upper physiological values (20 bar). The hyperphysiological pressures up to 40 bars result in partial tonoplast destruction, the pasmalemma barrier functions and cell viability being preserved. The correlation between plant growth processes after pressure treatment and increase in transmembrane water transfer and retaining of aquaporin water transport activity is found.

There is obtained experimental evidence in favor of transporting-distributing functions of endoplasmic reticulum based on the ability of the membrane of the latter to form close highly permeable contacts with other membranes inside a cell.

(Velikanov et al.,  Cell Tissue Biol. 2010, 2011; Velikanov et al.,  Biol. Bull., 2011; Velikanov, Biochemistry (Moscow) A: Membrane and Cell Biology (Biol. Membrany) 2009; Velikanov et al., Rus. J. Plant Physiol. 2005, 2008)

The efficiency of the cytoskeleton actomyosin motor in providing active water transport in a cytoplasm on the example of Elodea cells is registered in the form of an extreme temperature dependence of water diffusion coefficient. It is shown that the effect of the cytoskeleton on the water permeability of plasmodesmata and aquaporins can take place both via mechanical (steric) cytoskeleton functions, and via the level of hydration of cytoskeleton components in the course of their synthesis/degradation.

(Vorob’ev and Anisimov, Rus. J. Plant Physiol. 2010; Vorob’ev et al., Eur. Biophys. J. 2008; Vorob’ev et al., Protoplasma 2004; Anisimov et al., Doklady Biochem. Biophys. 2003)

The method is developed to obtain the maps of diffusional permeability of porous (bio)-systems based on the diffusion weighted MRI. For the first time there was obtained the map of diffusional permeability of intercellular water transport pathways in plant tissues. The difference between carrot tissues with respect to diffusional permeability of intercellular water transport pathways is determined. The anisotropy of diffusional permeability in carrot tissues is found.

(Sibgatullin et al., Eur. Biophys. J. 2010; Sibgatullin et al., Biophysics 2007)

Methodical developments 

A number of methodical and technical developments connected to the laboratory investigations are realized. Their main purpose is adaptation of the NMR technique to studies of intact plants (two-channel probe, equipment for mass transfer studies under the effect of static and dynamic pressure immediately in the probe, high stability system of temperature control within the physiological temperatures) and the increase in the sensitivity and stability of low field NMR systems, as well as modification of the NMR technique with pulsed magnetic field gradient in order to widen the range of available diffusion times (during the study of restricted diffusion in liquids) towards the ultra short times.

(Anisimov and Dautova, Rev. Sci. Instrum. 2010; Anisimov et al., Concepts Magn. Reson. Part B 2008; Anisimov, Instrum. Exp. Tech. 2009)

Selected Publications 2007-2013

  1. Ionenko, I.F., Dautova, N.R., Anisimov, A.V. Early changes of water diffusional transfer in maize roots under the influence of water stress. (2012) Environmental and Experimental Botany, 76, pp. 16-23.
  2. Ryazantseva I.N., Saakov V.S., Andreyeva I.N., Ogorodnikova T.I., Zyev Y.F. Response of pigmented serratia marcescens to the illumination. (2012) J. Photochemistry and Photobiology, B: Biology,106, pp.18–23.
  3. Velikanov, G.A., Ponomareva, A.A., Belova, L.P., Ilyina, T.M. Stromule-like protrusions of plastid membrane envelope in root cells. (2011) Cell and Tissue Biology, 5 (3), pp. 305-310.
  4. Velikanov, G.A., Belova, L.P., Ponomareva, A.A. Membrane contacts of the endoplasmic reticulum and their possible functions in the plant cell. (2011) Biology Bulletin, 38 (1), pp. 1-9.
  5. Beckett R.P., Alyabyev A.J., Minibayeva F.V. Patterns of heat production during desiccation and regydratation in lichens in desiccation tolerance. (2011) The Lichenologist,43(2), pp.178-183.
  6. Rakhmatullina D., Alyabyev A., Ogorodnikova T., Ponomareva A. Nitric oxide effect on heat production and oxygen consumption of wheat root cells. (2011) J. Thermal Analysis and Calorymetry, 104(1), pp. 157-160.
  7. Alyabyev A., Andreyeva I., Rachimova G. Influence of pH shift and salting on the energetics of microalgae Chlorella vulgaris and Dunaliella maritime. (2011) J. Thermal Analysis and Calorymetry, 104, pp. 201-207.
  8. Ionenko, I.F., Anisimov, A.V., Dautova, N.R. Effect of temperature on water transport through aquaporins. (2010) Biologia Plantarum, 54 (3), pp. 488-494.
  9. Velikanov, G.A., Ponomareva, A.A., Belova, L.P., Levanov, V.Y. Contacts of the endoplasmic reticulum membrane with plasmalemma in plant cells. (2010) Cell and Tissue Biology, 4 (2), pp. 199-207.
  10. Anisimov, A.V., Dautova, N.R. A simple air system for temperature stabilization in the range -10 to +80 °C for use in NMR spectroscopy. (2010) Review of Scientific Instruments, 81 (7), art. no. 075101.
  11. Vorob'ev, V.N., Anisimov, A.V. Hydrodynamics of cytoplasmic streaming and the cytoskeletal tubulin component in cells of Elodea densa internodes. (2010) Russian Journal of Plant Physiology, 57 (3), pp. 448-450.
  12. Anisimov, A.V. NMR stabilization of the magnetic field by a III1-1 magnetic inductometer in the frequency modulation mode. (2009) Instruments and Experimental Techniques, 52 (4), pp. 613-614.
  13. Velikanov, G.A. Stromules: Origin, structure and functions in a plant cell. (2009) Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 3 (4), pp. 395-403.
  14. Anisimov, A.V., Mishagina, E.A., Dautova, N.R. Simple modifications to the NMR PGSE technique for investigations in the regime of short diffusion observation times. (2008) Concepts in Magnetic Resonance Part B: Magnetic Resonance Engineering, 33 (4), pp. 216-219.
  15. Vorob'ev, V.N., Anisimov, A.V., Dautova, N.R. Water diffusion in cytoplasmic streaming in Elodea internodal cells under the effect of antimitotic agents. (2008) European Biophysics Journal, 37 (6), pp. 975-978.
  16. Velikanov, G.A., Belova, L.P., Levanov, V.Yu. Vacuolar symplast formation is due to highly permeable gap junctions between the tonoplast and endoplasmic reticulum membrane. (2008) Russian Journal of Plant Physiology, 55 (6), pp. 834-842.
  17. Ionenko, I.F., Anisimov, A.V. Differential sensitivities of water diffusion in maize root cortex and stele to HgCl2-induced blockade of aquaporins. (2008) Russian Journal of Plant Physiology, 55 (3), pp. 328-332.
  18. Ionenko, I.F., Anisimov, A.V. Radial diffusion transport of water in various zones of maize root and its sensitivity to mercury chloride. (2007) Russian Journal of Plant Physiology, 54 (2), pp. 224-229.
  19. Sibgatullin, T.A., De Jager, P.A., Vergeldt, F.J., Gerkema, E., Anisimov, A.V., Van As, H. Combined analysis of diffusion and relaxation behavior of water in apple parenchyma cells. (2007) Biophysics, 52 (2), pp. 196-203.
  20. Alyabyev A.Ju., Loseva N.L., Gordon L.Kh., Andreyeva I.N., Rachimova G.G., Tribunskih V.I., Ponomareva A.A., Kemp R.B. The effect of changes in salinity on the energy yielding processes of Chlorella vulgaris and Dunaliella maritima cells. (2007) Thermochimica Acta, 458(1-2), pp. 65-70.
  21. Loseva N.L., Alyabyev A.Ju., Gordon L.Kh., Andreyeva I.N. , Kolesnikov O.P., Ponomareva A.A., Kemp R.B. The effect of AgNO3 on the bioenergetic processes and the ultrastructure of Chlorella and Dunaliella cells exposed to different saline conditions. (2007) Thermochimica Acta, 458(1-2), pp. 71-76.
  22. Gordon L.K., Rakhmatullina D.F., Ogorodnikova T.I, Alyabyev A.J., Minibayeva F.V., Loseva N.L, Mityashina S.Y. The influence of ascorbic acid on the oxygen consumption and the heat production by the cells of wheat seedling roots with their mitochondrial electron transport chain inhibited at complexes I and III. (2007) Thermochimica Acta, 458(1-2), pp. 92-96.