Казанский институт биохимии и биофизики КазНЦ РАН


Laboratory of Physiology and Genetics of Cell Cultures

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The Laboratory was established in 2003 году on the base of group of experimental morphogenesis of the Laboratory of cell wall biochemistry

Head of Laboratory

Rumyantseva Natalya, Cand. Sc. (Biology),
e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Lab members:
Akulov Anton, Cand. Sc. (Biology), Senior Research Scientist, e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Gumerova Elena, Cand. Sc. (Biology), Research Scientist, e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Kostyukova Yulia, Cand. Sc. (Biology), Junior Research Scientist, e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Areas of current interest:

1. The secretome  and its role in the differentiation and adaptation of plant cells in vitro.

2. The role of redox homeostasis in the regulation of cell proliferation and morphogenesis in plant cell cultures.

3. Transcriptome and proteome analysis of callus cultures  of  tatar buckwheat, differing in  the morphogenic  activity. Identification of genes and proteins involved in the regulation of somatic embryogenesis.

4. The search for phenolic compounds with high antioxidant and regulatory activity in cultured cells of the Fagopyrum genus.

Selected publications (2003-2012):

  1. Rumyantseva N.I., Samaj J., Ensikat H.J., Sal'nikov V.V., Kostyukova Y.A., Baluska F., Volkmann D. Changes in the extracellular matrix surface network during cyclic reproduction of the proembryonic cell complexes in theFagopyrum tataricum (L.) Gaertn callus. // Dokl Biol Sci.- 2003.- V.391.- P.375-378.
  2. Gumerova E.A.  Galeeva, E.I. , Chuyenkova S.A.  , Rumyantseva N.I.  Somatic embryogenesis and bud formation on cultured Fagopyrum esculentum hypocotyls // Russian Journal of Plant Physiology.- 2003.- V.50.- P. 640-645.
  3. Rumyantseva N.I., Akulov A.N., Mukhitov A.R. Extracellular polymers in callus cultures of Fagopyrum tataricum(L.) Gaertn. with different morphogenic activities: time courses during the culture cycle // Applied Biochemistry and Microbiology.- 2004.- V.40.- I. 5.- P.494-500.
  4. Lebedeva V.V., Akulov A.N., Rumyantseva N.I. The cytological and biochemical peculiarities of the primary and secondary calli formation on maize root apices // Physiology and biochemistry of cultivated plants.- 2005.- V. 37.-I. 5.- P. 406-412.
  5. Rumyantseva N.I., Sal’nikov V.V., Lebedeva V.V. Structural changes of cell surface in callus of Fagopyrum esculentum Moench. during induction of morphogenesis // Russian Journal of Plant Physiology.- 2005.- V. 52.-I.3.- P. 381–387.
  6. Rumyantseva N.I. Arabinogalactan proteins: involvement in plant growth and morphogenesis)// Biochemistry (Mosc).- 2005.- V. 70.- I.10. -P. 1073-1085.
  7. Maksyutova N.N., Galeeva E.I., Rumyantseva N.I., Viktorova L.V. Proteins as morphogenetic markers in callus cultures of buckwheat Fagopyrum tataricum (L.) Gaertn with different morphogenetic potential // Biology Bulletin. RAS.- 2005.- V. 32.- I. 3.- P. 250-253.
  8. Maksyutova N.N., Galeeva E.I., Rumyantseva N.I., Viktorova L.V. Effect of salicylic acid on protein composition of tatar buckwheat Fagopyrum tataricum calluses with different ability for morphogenesis // Biochemistry (Mosc). - 2005.- V.70.- I.3З.- P. 316-321.
  9. Gumerova E.A., Utina D.B., Rumyantseva N.I. Establishment and Characterization of Morphogenic Suspension Culture of Tatar Buckwheat Fagopyrum tataricum (L.) Gaertn. // Uchenye Zapiski Kazanskogo Universiteta (Proceedings of Kazan University).- 2008.- V. 150.- book. 2.-  P. 126–135 (In Russian).
  10. Kamalova, G.V., Akulov, A.N., and Rumyantseva, N.I. Comparison of Redox State of Cells of Tatar Buckwheat Morphogenic Calluses and Non-Morphogenic Calluses Obtained from Them // Biochemistry (Mosc).- 2009.- V. 74.- P. 686–694.
  11. Sibgatullina G., Nigmatullina L., Rumyantseva N. The alteration of morphological, cytogenetical and biochemical characteristics of common buckwheat calli during long-term culture // Uchenye Zapiski Kazanskogo Universiteta (Proceedings of Kazan University).-  2009.- V. 151.- book 4.-  P. 103-112 (In Russian).
  12. Maksyutova N., Galeeva E., Mukhitov A., Rumyantseva N., Viktorova L. Changes in the Growth of Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn.) Calli with Different Ability for Morphogenesis Induced by Salicylic Acid // The European Journal of Plant Science and Biotechnology.- 2009.- V.3.- Special Issue 1.- Р.71-74.
  13. Rumyantseva N.I., Akulov A.N., Fedina E.O., Petrova N.V., Karimova F.G. Phosphorylation of proteins in the cultured buckwheat cells differing in morphogenic abilities // Russian Journal of Plant Physiology. 2010.- V.57.- I.1.- P.50-56.
  14. Akulov A.N., Rumyantseva N.I., Skripnikov A.Y. Expression of 1-cys peroxiredoxin in morphogenic and nonmorphogenic tatar buckwheat calli // Russian Journal of Plant Physiology.- 2010.- V. 57.- I.3.- P. 108–114.
  15. Kamalova G.V., Akulov, A.N., Rumyantseva, N.I. Two-dimensional gelelectrophoresis, carried out at non-denaturative conditions as an efficient method of study of plant antioxidant enzymes spectra // Agrarnaya Rossiya. – 2009. – Special Issue. – P. 86 (In Russian).
  16. Abdrakhimova I.R., Valieva A.I. The secondary plant metabolites: physiological and biochemical aspects. Part 3.Phenolic compounds // Manual to special course «The secondary metabolism of plants».- Kazan: Kazan (Volga Region) Federal University.- 2012. -P. 40.(In Russian)
  17. Akulov A.N., Gumerova E.A., Khaertdinova L.R., Kostyukova Yu.A., Nikonorova N.A., Rumyantseva N.I., Sibgatullina G.V. Methods for determining the redox-state of plant cell cultures: manual // Kazan: Kazan (Volga Region) Federal University.- 2012. –P. 61. (In Russian).
  18. Sibgatullina G. V., Rumyantseva N. I., Khaertdinova L. R., Akulov A. N., Tarasova N. B., Gumerova E. A. Establishment and characterization of the line of Fagopyrum tataricum morphogenic callus tolerant to aminotriazole // Russian Journal of Plant Physiology.- 2012.- V. 59.- I. 5.-P. 662–669.
  19. Timofeeva O.A., Rumyantseva N.I. Plant cell and tissue culture. // Text book. Kazan: Kazan (Volga Region) Federal University.- 2012. –P. 61-91. (In Russian).
  20. Naumenko E.A., Sibgatullina G.V., Mukhitov A.R., Rodionov А.А., Ilinskaya О.N., Naumova R.P. 2,4,6-trinitrotoluene – trigger of oxidative stress in cells of Fagopyrum tataricum callus// Russian Journal of PlantPhysiology. 2013. V.60, №3. P.416-423.
  21. Nigmatullina L.R., Rumyantseva N.I., Kostyukova Yu.A. Effect of D,L -buthionine -S,R- sulfoximine on the ratio of glutathione forms and the growth of tatar buckwheat calli// Russian Journal of Developmental Biology. 2014. V. 45, № 1. P. 41–51.


Different  methods of culture of plant cells, tissues and organs (the establishment of callus and suspension cultures, plant regeneration, micropropagation, isolation and culture of plant protoplasts, embryo culture); histological, cytochemical, cytogenetic analysis, electron microscopy; various methods of proteomic analysis (one-dimensional and two-dimensional gel electrophoresis, the identification of proteins by MALDI-TOF MS, electrophoretic and spectrophotometric methods for the analysis of various enzymes, immunoblotting); determination of redox status of cultured cells (hydrogen peroxide and nitric  oxide, oxidized and reduced forms of glutathione, the activity of various antioxidant enzymes); methods to determine the content of phenolic compounds and their antioxidant activity, HPLC analysis of qualitative composition of phenolic compounds; PCR, RT-PCR.


RFBR, 05-04-49433_a (2005-2007) (Rumyantseva N.I. Principal Investigator)
09-04-90756-mob_st (2009)  (Rumyantseva N.I. Principal Investigator)

The grant of President of Russian Federation for State Foundation of Young Russian Scientists, МК-4249.2009.4 (2009-2010) (Akulov A.N. Principal Investigator)

RFBR, 09-04-97039-a (2009-2011) (Rumyantseva N.I. Principal Investigator)

RFBR, 12-04-31006-mol_а (2012-2013) (Sibgatullina G.V. Principal Investigator)

RFBR, № 13-04-06067_g (2013) (Rumyantseva N.I. Principal Investigator) 

RFBR, №13-04-97136_ а (2013-2014) (Rumyantseva N.I. Principal Investigator)