The Laboratory of Molecular Bases of Pathogenesis

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Head of the Laboratory

Chernov Vladislav M.
D.Sc. (Protection of Plants), professor.
Tel.: (843)2927347, (843)2319026
e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it









Lab members:

1. Chernova Olga A., D.Sc. (Biochemistry), professor, This e-mail address is being protected from spambots. You need JavaScript enabled to view it

2. Davydova Marina N., Cand.Sc.,  This e-mail address is being protected from spambots. You need JavaScript enabled to view it
3. Ryazantseva Irina N., Cand.Sc.,  This e-mail address is being protected from spambots. You need JavaScript enabled to view it
4. Shaymardanova Gulnara F., Cand.Sc.,  This e-mail address is being protected from spambots. You need JavaScript enabled to view it  
5. Trushin Maxim V., Cand.Sc.,  This e-mail address is being protected from spambots. You need JavaScript enabled to view it  
6. Nesterova Tatyana N., Cand.Sc., This e-mail address is being protected from spambots. You need JavaScript enabled to view it  
7. Mouzykantov Alexey A., Cand.Sc., This e-mail address is being protected from spambots. You need JavaScript enabled to view it
8. Baranova Natalia B., Cand.Sc., This e-mail address is being protected from spambots. You need JavaScript enabled to view it
9. Medvedeva Elena S., Cand.Sc., This e-mail address is being protected from spambots. You need JavaScript enabled to view it
10. Grigoryeva Tatyana  This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Historical Background

In June 1987, a Group of Molecular Genetics of Plant Mycoplasmas was established at the Institute owing to the initiative of academician I.A. Tarchevsky; in June 1988, this Group was transformed into the Laboratory of Molecular Genetics of Mycoplasmas. The Declaration of the Central Committee of Communist Party of the Soviet Union and the USSR Cabinet of Ministry of August 26, 1985 (#807) – “On further development of new directions in biology and biotechnology” formed the basis for the foundation of a new Laboratory and initiation of research of the molecular-genetic fundamentals of interaction between plants and pathogenic microorganisms within the Institute.

The choice of mycoplasmas as a model of pathogenic microorganisms was defined by the unicity of organization of the smallest prokaryotes the limited biosynthetic abilities of which did not prevent their broad distribution in the nature, overcoming the defense systems of higher eukaryotes and persistence. Much attention to mycoplasmas is given because of the practical necessity: mycoplasmas are parasites of humans, animals, plants, and main contaminants of cell cultures widely used in biotechnology. Control of mycoplasma infections and contaminations presents a serious problem the resolution of which is connected with the advantages of investigation of interaction between mycoplasmas and higher eukaryotes, and adaptation of these microorganisms to stressors.

The first researchers of the Laboratory were V.M. Chernov and O.A. Chernova (post-graduate students of the Institute of Cytology, USSR Academy of Sciences, Leningrad), M.A. Khasanova (post-graduate student of the Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Puschino-na-Oke) and an engineer Yu.V. Gogolev (from the Laboratory of Biochemistry of Cell Wall under Prof. V.V. Lozovaya).

In 2002 the Laboratory was renamed as the Laboratory of Molecular Fundamentals of Pathogenesis. In 2003, a group of microbiologists headed by Dr. M.N. Davydova was incorporated into the Laboratory. Previously, the scientific workers of the group formed the main part of the Laboratory of Physiology and Biochemistry of Anaerobic Bacteria, founded in 1976 by an eminent professor M.I. Belyaeva. In 2006, a part of the researchers joined a Group of Molecular Biology headed by Dr. Yu.V. Gogolev.

Much of the scientific work of the Laboratory is related to the investigation of molecular and cell biology of mycoplasmas that infect humans, animals, plants, the main contaminants of cell cultures and vaccines.

The success of the genome projects for mycoplasmas determined the feasibility of comparative proteomic profiling of these microorganisms cultivated under stress conditions as well as of higher organisms with mycoplasmas. Studies on the genome-transcriptome-proteome analysis of mycoplasmas and eukaryotes are supplemented with the investigations of the ultrastructure of cells interacting organisms.

The basic research topic of the Laboratory is the interaction between mycoplasmas and eukaryotes: the analysis of genomes’, proteomes’, transcriptoms’. 

Basic results for 2007-2012:

Applied research:

The investigations on technologic application of bacterial pigments in polymer coloring in chemical industry conducted as a result, the method for utilization of bacterial pigments in technology of polyoleofine coloring has been developed and patented. Synthesis of microbial pigments was performed using intermediates of cell metabolism. It excludes expenses for multistage synthesis of pigment molecule and is recognized as the ecologically pure process.

Work on personnel training:

Under the guidance of the Laboratory staff for 2003-2012, the following Cand.Sc. dissertations were defended:

Collaboration with:

Basic publications for 2003-2012: 

  1. Arleevsky I.P., Chernova O.A., Ganeeva L.A., Saphin I.N., Chernov V.M. Mycoplasma infections and myocardial infarction // Ross. Cardiol. Zhurn.-2003.-#4.-P.17-23.
  2. Chernov V.M., Gogolev Y.V., Mukhametshina N.E., Abdrakhimov F.A., Chernova O.A. Mycoplasma adaptation to biogenic and abiogenic stressful factors; Acholeplasma laidlawii nannotransformation and minibodies // Dokl. Biol. Sci. – 2004. – Vol. 396. – P. 251-254.
  3. Chernov V.M., Mukhametshina N.E., Gogolev Yu.V., Abdrakhimov F.A., Chernova OA. Adaptive reactions of mycoplasmas in vitro: “viable but unculturable forms” and nanocells of Acholeplasma laidlawii // Mikrobiologiia. – 2005. – Vol. 74, N 4. – P. 498-504.
  4. Chernov V.M., Govorun V.M., Demina I.A., Gorshkov O.V., Muzykantov A.A., Shaimardanova G.F., Chernova O.A. Adaptation of mycoplasmas to adverse growth conditions: morphology, ultrastructure, and genome expression of Mycoplasma gallisepticum S6 cells // Dokl. Biochem. Biophys. – 2008. – Vol. 421. – P. 231-234.
  5. Abuzarova E.R., Gorshkov O.V.,  Chernova O.A., Chernov V.M., Akberova N.I., Abdulkhakov R.A. Features of distribution of interleukine genotypes (IL-1 and IL-10) in patients with ulcer disease and their association with persistence of Mycoplasma hyorhinis and genotypes of Helicobacter pylori // Experimental and clinical gastroenterology.-2008.-№6.-p.27-31.
  6. Chernova O.A., Baranova N.B., Akberova N.I., Gorshkov O.V., Mouzykantov A.A., Chernov V.M. Distribution of frequencies of alleles and genotypes of polymorphic loci IL-1B, IL-1RN, and IL-10 in humans at mycoplasma persistence (Mycoplasma hominis) // Cytokines and inflammation.-2008.-V.7.#4.- p.11-14.
  7. Chernov V.M., Chernova O.A., Gorshkov O.V., Mouzykantov A.A. Adaptation of mycoplasmas to stressful factors: nucleotide sequences nonregistered in vegetative foms M. gallisepticum S6 cells are revealed in nonculturable forms of the mycoplasma // Mol. Biol. (Mosk). – 2009. – Vol. 43, N 4. – P. 642-647.
  8. Chernov V.M., Chernova O.A., Medvedeva E.S., Sorvina A.I., Davydova M.N., Rogova M.A., Serebryakova M.V. Responses of Acholeplasma laidlawii PG8 cells to cold shock and oxidative stress: proteomic analysis and stress-reactive mycoplasma proteins // Dokl. Biochem. Biophys. – 2010. – Vol. 432, N 5. – P. 126-130.
  9. Chernov V.M., Chernova O.A., Baranova N.B., Gorshkov O.V., Medvedeva E.S., Shaymardanova G.F. Mycoplasma adaptation to stress conditions: proteome shift in Mycoplasma hominis PG37 in response to starvation and low temperatures // Mol. Biol. (Mosk). – 2011. – Vol. 45, N 5. – P. 843-851.
  10. Chernov V.M., Gorshkov O.V., Chernova O.A., Baranova N.B., Akopian T.A., Trushin M.V. Variability of the vaa cytoadhesin genes in clinical isolates of Mycoplasma hominis // NEW Microbiol. – 2005. – Vol. 28. – P. 373-376.
  11. Chernov V.M., Moukhametshina N.E., Gogolev Y.V., Nesterova T.N., Trushin M.V., Chernova O.A. Differential amplification of Acholeplasma laidlawii PG8 rrnA and rrnB nucleotide sequences during dissociation of the cell culture population// Journal of Rapid Methods and Automation in Microbiology. 2006. 14: 369-376.
  12. Chernova O.A., Trushin M.V., Mouzykantov A.A., Chernov V.M. Atomic force microscopy investigation of DNA extracted from the vegetative forms and the viable but nonculturable forms of Mycoplasma gallisepticum S6 // TheScientificWorldJOURNAL. – 2008. – Vol. 8. – P. 1104-1110.
  13. Chernov V.M., Chernova O.A., Mouzykantov A.A., Ponomareva A.A., Trushin M.V., Gorshkov O.V., Nesterova T.N. Phytopathogenicity of avian mycoplasma Mycoplasma gallisepticum S6: morphologic and ultracytostructural changes in plants infected with the vegetative forms and the viable but nonculturable forms of the bacterium // Microbiol. Res. – 2010. – Vol. 165, N 4. – P. 346-350.
  14. Trushin M.V., Chernov V.M., Gorshkov O.V., Baranova N.B., Chernova O.A. Atomic force microscopy analysis of DNA extracted from the vegetative cells and the viable, but nonculturable, cells of two mycoplasmas (Acholeplasma laidlawii PG8 and Mycoplasma hominis PG37) // TheScientificWorldJOURNAL. – 2010. – Vol. 10. – P. 894-900.
  15. Chernov V.M., Chernova O.A., Mouzykantov A.A., Efimova I.R., Shaymardanova G.F., Medvedeva E.S., Trushin M.V. Extracellular vesicles derived from Acholeplasma laidlawii PG8 // TheScientificWorldJOURNAL. – 2011. – Vol. 11. – P. 1120-1130.
  16. Chernov V.M., Chernova O.A., Medvedeva E.S., Mouzykantov A.A., Ponomareva A.A., Shaymardanova G.F., Gorshkov O.V., Trushin M.V. Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis // J. Proteomics. – 2011. – Vol. 74, N 12. – P. 2920-2936.
  17. Chernov V.M., Chernova O.A., Mouzykantov A.A., Baranova N.B., Gorshkov O.V., Trushin M.V., Nesterova T.N., Ponomareva A.A. Extracellular membrane vesicles and phytopathogenicity of Acholeplasma laidlawii PG8 // TheScientificWorldJOURNAL, vol. 2012, Article ID 315474, 6 pages.

 

Monographs:

Patents and certificates of autorship:

  1. Borkhsenius S.N., Chernova O.A., Merkulova N.А., Smirnova Т.D. A way for detection of mycoplasma infections / certificate of autorship № 1374783. 1987.
  2. Borkhsenius S.N., Merkulova N.А., Chernova O.A. Recombinant plasmid DNA pMg16 for detection of mycoplasma infections / certificate of autorship № 1413960. 1988.
  3. Borkhsenius S.N., Chernova O.A., Merkulova N.А., Chernov V.М. A way for identification of Mycoplasma orale in cells cultured in vitro / certificate of autorship № 1559710. 1989.
  4. Chernov V.М., Khasanova М.А., Chernova О.A. Recombinant plasmid DNA рА17 used as DNA probe for detection of Acholeplasma laidlawii 118 in plant cells / certificate of autorship № 1835851. 1992.
  5. Chernova О.A., Chernov V.М., Merkulova N.А., Borkhsenius S.N. A way for identification of Mycoplasma fermentas in cells cultured in vitro / certificate of autorship № 1559709. 1989а.
  6. Chernova О.A., Chernov V.М., Merkulova N.А., Borkhsenius S.N., Shagimukhametova F.F. A way for identification of Mycoplasma salivarium in cells cultured in vitro / certificate of autorship № 1559708. 1989b.
  7. Chernova O.A., Khasanova М.А., Chernov V.М. Recombinant plasmid DNA рА1S6, used as DNA probe for detection of Acholeplasma laidlawii 185 in plant cells / certificate of autorship № 1835852. 1992.
  8. Gogolev Yu.V., Avzalov F.Z., Idrisov G.Z., Chernov V.M., Gaffarov Kh.Z. A method of detection of infected with Chlamydia psittaci agricultural animals / certificate of autorship № 2135516. 1999.
  9. Kudryashov V.N., Ryazantseva I.N., Bobreshova E.E., Andreeva I.N., Valieva N.N., Ogorodnikova T.I. Polymeric coloring composition / Patent № 2420541. 2009.

Grants: